Table 1 Phenotype and length of the inserted marker fragment in the eight ∆trp3 strains
From: Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein
Strain | Phenotype*1 | Fragment length*2 | |||
|---|---|---|---|---|---|
Ura | Trp | PCR*3 | Sequenced*4 | ||
STRB012 | P | P | NA | NA | |
STRB018 | A | P | NA | NA | |
∆trp3_c1_1 | P | A | 2130 bp | + | 1955 bp |
∆trp3_c1_2 | P | A | 270 bp | + | 0 bp*5 |
∆trp3_c1_3 | P | A | 2880 bp | + | 2962 bp |
∆trp3_c1_4 | P | A | 0 bp | – | NA |
∆trp3_c1_5 | P | A | 2370 bp | + | 2380 bp |
∆trp3_c2_1 | P | A | 1900 bp | – | NA |
∆trp3_c2_2 | P | A | 990 bp | – | NA |
∆trp3_c2_3 | P | A | 2250 bp | – | NA |