Skip to main content
Fig. 5 | BMC Biotechnology

Fig. 5

From: Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

Fig. 5

Transformation and analysis of T. rubrum ∆trp3. a and b One month old transformation plates. Control plates (left) without colonies, transformation with sgRNA construct 1 (middle) and transformation with sgRNA construct 2 (right). c and d. Auxotrophy test of strain 1–3 of construct 1 on SC medium without tryptophan. The wild-type strain STRB012 grown well whereas ∆trp3 strains c1.1-c1.3 are auxotrophic for tryptophan

Back to article page