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Fig. 3 | BMC Biotechnology

Fig. 3

From: Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

Fig. 3

Transformation and analysis of T. rubrum ∆ura3 strains. a Three weeks old transformation plate supplemented with FOA for counter selection. ∆ura3 strains are visible as distinct colonies. b Three weeks old control plate supplemented with FOA for counter selection. c, d Auxotrophy test of ∆ura3 and parent strain. SC medium supplied with uracil and uridine. The wild-type and the ∆ura3 strain have a similar growth rate e, f. Auxotrophy test of ∆ura3 and parent strain. SC medium without uracil and uridine. Only the wild-type strain is growing. g DNA sequence alignment of the mutated part of the URA3 gene. Top sequence: Part of the protospacer sequence including PAM sequence. Middle: Auxotrophic strain STRB018. Bottom: Auxotrophic strain STRB019

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