Table 1 Oligos used for introduction of gRNA sequences for TRAC and CD52 genes into pSpCas9-2A-GFP plasmid
From: CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells
Name | Sequence |
|---|---|
TRAC-gRNA (F/top) TRAC-gRNA (R/bottom) | 5′p-CACCGTCTCTCAGCTGGTACACGGC-3′ 5′p-AAACGCCGTGTACCAGCTGAGAGAC-3′ |
CD52-gRNA (F/top) CD52-gRNA (R/bottom) | 5′p-CACCGCAGCCTCCTGGTTATGGTAC-3′ 5′p-AAACGTACCATAACCAGGAGGCTGC-3′ |