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Table 1 Effect of various chemical agents on keratinase stability

From: Bacillus sp. CSK2 produced thermostable alkaline keratinase using agro-wastes: keratinolytic enzyme characterization

Chemical agent Concentration Residual activity (%)
None 100 ± 4.24d
EDTA 5 mM 0 ± 0a
1,10-phenanthroline 5 mM 0 ± 0a
PMSF 5 mM 89 ± 6.22c
Sodium thioglycolate 5 mM 64 ± 2.70b
Dithiothreitol 5 mM 69 ± 0.09b
Sodium sulfite 5 mM 78 ± 3.61c
2-Mercaptoethanol 5 mM 111 ± 0.54e
Hydrogen peroxide 1% (v/v) 103 ± 3.52d
Dimethyl sulfoxide 1% (v/v) 114 ± 5.86e
Acetonitrile 1% (v/v) 61 ± 3.34b
Propan-2-ol 1% (v/v) 79 ± 3.97c
Sodium dodecyl sulfate 5 mM 77 ± 4.51c
Triton X-100 1% (v/v) 99 ± 2.16d
Tween-80 1% (v/v) 182 ± 1.98f
  1. Superscript letter(s) a, b, c, d, e and f down the column were used to indicate significant difference at P < 0.05
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