Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro

Table 1 Comparison of decellularization and cytotoxicity of decellularization methods

Decellularization methods		SDS 18 Mean ± SD	SDS24 Mean ± SD	SDS-T Mean ± SD	SDS-T-A Mean ± SD
Residual DNA (%)		10.4 ± 2.35 ^a	9.13 ± 0.85 ^b	15.2 ± 1.9 ^c	7.16 ± 1.6 ^d
MTT test assay (%)	24 Hours	87.33% ± 28.57	68% ± 20.51	80% ± 9.84	96.33% ± 12.74
MTT test assay (%)	7 days	43.33 ± 10.50 ^e	14% ± 8.32 ^f	77.66% ± 14.64 ^g	75% ± 15.71 ^h

Significant difference between a and c (P < 0.028), b and c (P < 0.054), c and d (P < 0.011), e and f (P < 0.03), e and g (P < 0.014), e and h (P < 0.021), f and g (P < 0.001), and f and h (P < 0.001). The experiments where performed in three replication and results were expressed as mean ± standard deviation

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