Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

Table 3 Box-Behnken design for xylanase production by Bacillus aestuarii UE25

Run Order	Temperature (°C)	Incubation time (h)	pH	Agitation (rpm)	Inoculum size (%)	Xylanase production (IU mL^− 1)^a
1	55	24	6	75	7.5	0
2	60	24	6	75	7.5	14.20
3	55	72	6	75	7.5	7.13
4	60	72	6	75	7.5	9.15
5	57.5	48	5	0	7.5	6.69
6	57.5	48	7	0	7.5	17.95
7	57.5	48	5	150	7.5	13.32
8	57.5	48	7	150	7.5	11.79
9	57.5	24	6	75	5	11.70
10	57.5	72	6	75	5	9.59
11	57.5	24	6	75	10	12.41
12	57.5	72	6	75	10	12.76
13	55	48	5	75	7.5	0
14	60	48	5	75	7.5	6.74
15	55	48	7	75	7.5	6.86
16	60	48	7	75	7.5	9.33
17	57.5	48	6	0	5	10.12
18	57.5	48	6	150	5	20.17
19	57.5	48	6	0	10	30.10
20	57.5	48	6	150	10	12.76
21	57.5	24	5	75	7.5	7.74
22	57.5	72	5	75	7.5	8.09
23	57.5	24	7	75	7.5	8.36
24	57.5	72	7	75	7.5	7.39
25	55	48	6	0	7.5	11.03
26	60	48	6	0	7.5	14.96
27	55	48	6	150	7.5	11.53
28	60	48	6	150	7.5	22.26
29	57.5	48	5	75	5	8.85
30	57.5	48	7	75	5	8.80
31	57.5	48	5	75	10	9.76
32	57.5	48	7	75	10	6.92
33	55	48	6	75	5	0
34	60	48	6	75	5	16.54
35	55	48	6	75	10	15.84
36	60	48	6	75	10	12.76
37	57.5	24	6	0	7.5	11.35
38	57.5	72	6	0	7.5	16.90
39	57.5	24	6	150	7.5	8.69
40	57.5	72	6	150	7.5	16.66
41	57.5	48	6	75	7.5	6.87
42	57.5	48	6	75	7.5	8.09
43	57.5	48	6	75	7.5	5.87
44	57.5	48	6	75	7.5	7.92
45	57.5	48	6	75	7.5	8.62
46	57.5	48	6	75	7.5	7.50

ISSN: 1472-6750