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Table 3 Box-Behnken design for xylanase production by Bacillus aestuarii UE25

From: Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

Run Order Temperature (°C) Incubation time (h) pH Agitation (rpm) Inoculum size (%) Xylanase production (IU mL− 1)a
1 55 24 6 75 7.5 0
2 60 24 6 75 7.5 14.20
3 55 72 6 75 7.5 7.13
4 60 72 6 75 7.5 9.15
5 57.5 48 5 0 7.5 6.69
6 57.5 48 7 0 7.5 17.95
7 57.5 48 5 150 7.5 13.32
8 57.5 48 7 150 7.5 11.79
9 57.5 24 6 75 5 11.70
10 57.5 72 6 75 5 9.59
11 57.5 24 6 75 10 12.41
12 57.5 72 6 75 10 12.76
13 55 48 5 75 7.5 0
14 60 48 5 75 7.5 6.74
15 55 48 7 75 7.5 6.86
16 60 48 7 75 7.5 9.33
17 57.5 48 6 0 5 10.12
18 57.5 48 6 150 5 20.17
19 57.5 48 6 0 10 30.10
20 57.5 48 6 150 10 12.76
21 57.5 24 5 75 7.5 7.74
22 57.5 72 5 75 7.5 8.09
23 57.5 24 7 75 7.5 8.36
24 57.5 72 7 75 7.5 7.39
25 55 48 6 0 7.5 11.03
26 60 48 6 0 7.5 14.96
27 55 48 6 150 7.5 11.53
28 60 48 6 150 7.5 22.26
29 57.5 48 5 75 5 8.85
30 57.5 48 7 75 5 8.80
31 57.5 48 5 75 10 9.76
32 57.5 48 7 75 10 6.92
33 55 48 6 75 5 0
34 60 48 6 75 5 16.54
35 55 48 6 75 10 15.84
36 60 48 6 75 10 12.76
37 57.5 24 6 0 7.5 11.35
38 57.5 72 6 0 7.5 16.90
39 57.5 24 6 150 7.5 8.69
40 57.5 72 6 150 7.5 16.66
41 57.5 48 6 75 7.5 6.87
42 57.5 48 6 75 7.5 8.09
43 57.5 48 6 75 7.5 5.87
44 57.5 48 6 75 7.5 7.92
45 57.5 48 6 75 7.5 8.62
46 57.5 48 6 75 7.5 7.50
  1. a With insignificant standard deviation
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