Skip to main content

Table 1 Plackett Burman experimental design (PBD) for xylanase production by using different pretreated sugarcane bagasse

From: Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

Run Order Bagasse pretreatment Medium* Incubation temperature (°C) pH Substrate concentration(%) Inoculumsize (%) Incubation time (h) Agitation (150 rpm)
  PBD 1 PBD 2 PBD 3        
1 NaOH+ILa NaOHb H2O2c MSM + 0.5% G 60 5 1 5 48 without
2 NaOH+IL NaOH H2O2 MSM + 0.5 G & P 55 7 1 5 24 without
3 ILd H2SO4e UTBf MSM + 0.5 G & P 60 5 2 5 24 with
4 NaOH+IL NaOH H2O2 MSM + 0.5% G 60 7 1 10 24 with
5 NaOH+IL NaOH H2O2 MSM + 0.5 G & P 55 7 2 5 48 with
6 NaOH+IL NaOH H2O2 MSM + 0.5 G & P 60 5 2 10 24 without
7 IL H2SO4 UTB MSM + 0.5 G & P 60 7 1 10 48 with
8 IL H2SO4 UTB MSM + 0.5% G 60 7 2 5 48 without
9 IL H2SO4 UTB MSM + 0.5% G 55 7 2 10 24 without
10 NaOH+IL NaOH H2O2 MSM + 0.5% G 55 5 2 10 48 with
11 IL H2SO4 UTB MSM + 0.5 G & P 55 5 1 10 48 without
12 IL H2SO4 UTB MSM + 0.5% G 55 5 1 5 24 with
  1. * MSM Mineral salt medium, G Glucose, P Peptone
  2. a = alkali + ionic liquid, b = alkali, c = hydrogen peroxide, d = ionic liquid, e = acid, f = untreated bagasse.
\