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Table 2 Primers and probes used for plasmid construction and real-time quantitative PCR

From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

 

Sequence (5′ → 3′)

Annotation

fib-F

GAATTCGCAGGGAAATCAAACGGG

PCR for pPIC9K-fib

fib-R

GCGGCCGCTTAGTGGTGATGATGGTGATGTTGAGCAGCAGCTTGAACGT

PCR for pPIC9K-fib

fib-F

GCAGGGAAATCAAACGGG

PCR for pMD19T-fib

fib-R

TTGAGCAGCAGCTTGAACGT

PCR for pMD19T-fib

TDH1-F

ATGGCTATCACTGTCGGTATTAACG

PCR for pMD19T-TDH1

TDH1-R

TTAAGCCTTAGCAACGTGTTG

PCR for pMD19T-TDH1

qfib-F

TTGCCGGTGGAGCTTCTATG

qPCR for fib

qfib-R

CAACAGAGTTGTTCAAAGCAGCA

qPCR for fib

Probe-fib

HEX-TCCATTCCAAGATTACAACTCTCATGG-BHQ-1

qPCR for fib

qTDH1-F

CGGTGTTTTCACCACTTTGGA

qPCR for TDH1

qTDH1-R

CAACGAACATTGGAGCATCCT

qPCR for TDH1

Probe-TDH1

HEX-CCAAAAGCACATCGACGCCGGT-BHQ-1

qPCR for TDH1