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Fig. 6 | BMC Biotechnology

Fig. 6

From: Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)

Fig. 6

Localization of HSD17β1, ADAMTS1, EDN2, and OVGP1. The ovaries were induced to superovulate with 10 IU of either N-eCG or R-eCGβ/α, followed by 10 IU of hCG after 48 h. Representative immunohistochemical analyses for HSD17β1, ADAMTS1, EDN2, and OVGP1 were conducted with antisera, and a goat anti-rabbit IgG antibody (secondary antibody). According to the microarray and qRT-PCR results, HSD17β1 was up-regulated in the R-eCG-treated ovaries, while the other three proteins (ADAMTS1, EDN2, and OVGP1) were up-regulated in the N-eCG-treated ovaries. Immunohistochemistry was performed with a Vectastain ABC kit. Scale bar = 200 μm

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