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Fig. 2 | BMC Biotechnology

Fig. 2

From: Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)

Fig. 2

Quantitative analysis and western blot analysis of tethered R-eCG. a Quantities of myc-tagged R-eCG mutants were analyzed by an ELISA. The myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) was inserted between the first and second amino acid residue of the β-subunit of mature eCG protein. b Western blot analysis of tethered R-eCG. The proteins in conditioned media were separated via SDS-PAGE and electro-transferred to a blotting membrane. The proteins were detected with antibodies against the myc-tag. Proteins for western blotting were also treated with N-Glycosidase F. -: not treated, +: treated with N-Glycosidase-F

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