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Fig. 5 | BMC Biotechnology

Fig. 5

From: Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Fig. 5

Purity and biotinylation assessment of SAG1bio-His6 and SAG2Abio-His6. a Silver staining of SDS-PAG of purified proteins. b Western blot analysis (same protein amounts as in A) with anti-His6 antibody to detect the proteins, or c, streptavidin, both coupled to horseradish peroxidase. The “bleached” signal for SAG2Abio-His6 in C was due to very strong chemiluminescence. Images of gels and blots were cropped; full-length blots/gels are presented in Additional file 8: Supplementary Fig. S2

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