Fig. 1

Sequence comparison between the mature forms of SAG1 and SAG2A. The residue numbering is according to the full-length, unprocessed proteins, whereas only the sequences without N- and C-terminal topogenic sequences are displayed. The sequence identity is 24.3% and similarity 34.1%, respectively. All cysteines are highlighted in yellow. Matching colors of the boxes in each sequence indicate the residues involved in the respective disulfide bond and are connected by lines. The three matching Cys pairs of SAG2A were inferred from [17]. The amino acids in each monomer of SAG1 forming the epitope are underlined according to [30]. It consists of Thr₆₇-Ala₆₈-Leu₆₉, Glu₇₁, Pro₇₃-Thr₇₄, Tyr₇₇, Asn₈₀, Gln₈₂ and Ser₉₁-Cys₉₂-Thr₉₃-Ser₉₄-Lys₉₅-Ala₉₆-Val₉₇, all part of a loop. In a second much shorter loop of the structure there are three consecutive residues, Ile₁₄₄-Lys₁₄₅-Gly₁₄₆, that are part of the epitope. No data for SAG2A exists in this respect