Table 6 Specific SSR and SCAR primers used for detection of CMD2 resistant gene in cassava mother plants and regenerated plantlets
From: In vitro propagation of three mosaic disease resistant cassava cultivars
Primer code | Marker system | Forward primer sequence | Reverse primer sequence | Expected sequence length (bp) | Annealing Temperature (°C) |
|---|---|---|---|---|---|
NS169 | SSR | GTGCGAAATGGAAATCAATG | GCCTTCTCAGCATATGGAGC | 319 | 55 |
RME1 | SCAR | AGAAGAGGGTAGGAGTTATGT | ATGTTAATGTAATGAAAGAGC | 700 | 55 |