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Table 2 Placket-Burman design matrix for determination of optimum conditions for PL inhibitor production by AspsarO

From: Kojic acid repurposing as a pancreatic lipase inhibitor and the optimization of its production from a local Aspergillus oryzae soil isolate

Runs

Factors

Responsec

Incubation Temperature

(°C)

Production time in Days

Carbon source replacing dextrose in PDB

Nitrogen source

% PL inhibition ± SD

1

25

5

sucrose

YE a

44.8 ± 3.3

2

37

5

sucrose

YE

48.0 ± 2.6

3

25

6

sucrose

YE

50.0 ± 2.6

4

37

6

sucrose

YE

53.1 ± 4.1

5

25

5

starch

YE

47.9 ± 1.4

6

37

5

starch

YE

46.1 ± 1.1

7

25

6

starch

YE

56.4 ± 0.9

8

37

6

starch

YE

48.0 ± 1.2

9

25

5

sucrose

TSB b

48.2 ± 3.3

10

37

5

sucrose

TSB

38.9 ± 0.9

11

25

6

sucrose

TSB

51.7 ± 5.7

12

37

6

sucrose

TSB

56.6 ± 2.0

13

25

5

starch

TSB

25.9 ± 2.4

14

37

5

starch

TSB

51.2 ± 0.7

15

25

6

starch

TSB

57.5 ± 2.3

16

37

6

starch

TSB

61.4 ± 1.8

17

25

5

–

–

43.3 ± 8.0

  1. a yeast extract, b tryptic soy broth. cAll runs were performed in triplicates; the response is represented as the mean of three independent experiments ± the standard deviation