Fig. 7
Quantitative RT-PCR analysis of the diesel inducible genes over time in A. calcoaceticus CA16. Cells with a starting OD600 of 0.5 were treated with 1% diesel or 0.1% glycerol (control). For each time point, expression of the diesel-activated genes encoding AlkM, EstB, OmpA, Wzc and XcpR (Genbank accession numbers AQZ81143, AQZ80440, AQZ80868, AQZ80173, and AQZ80682,respectively) were quantified relative to their expression in glycerol control samples. The mean expression values of four technical replicates per gene target were normalized to 16S rRNA expression. Results are presented as mean relative normalized expression ± SE of three biological replicates (n = 3)