Fig. 6From: One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodiesCharacterization of NM nanoparticles. The NM nanoparticles formation was processed through salting out with potassium phosphate. The NM nanoparticles were completely washed with pure water and air-dried on a gold-coated silicon, and subsequently observed under Hitachi scanning electron microscope (Japan, S-4700) with HV as 15 kV. The nanoparticles were magnified 5000× (a) and 10,0000× (b) One hundred microliters NM solution (2.5 mg/mL) was mixed with 1 mL 2 M potassium phosphate buffer at a pH of 8.0 and was incubated at room temperature for 2 h.Back to article page