Fig. 4

pH Effects on NM IBs solubilization with one-step heating method. The equal amount of purified NM IBs was suspended in 1 mL 10 mmol/L Tris-HCl at different pHs (range from 5.0 to 10.0) in the presence of 4 mol/L urea. a SDS-PAGE analysis of the effects of pH on NM IBs solubilization. b The quantification of solubilization efficiency at different pHs