Fig. 3

Effects of heating temperature on the solubilization of NM IBs with one-step heating method. Equal amount of purified NM IBs was suspended in 1 mL 10 mmol/L Tris-HCl at pH 8.0 containing 4 mmol/L urea and heating at different temperatures (40–100 °C). a SDS-PAGE analysis of the effect of heating temperature. b The quantification of solubilization efficiency at different heating temperature