Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

Fig. 1

Amino acid sequence, plasmid construction and NM expression. a Amino acid sequence of NM protein derived from the A. ventricosus MiSp. The NM sequence consists of the NT domain (161-aa, in green) and a truncated repetitive region (261-aa, in blue), between which there is an enterokinase cleavage site (DDDDK). b The gene fragment coding for NM was inserted into pET-32a plasmid within NdeI and XhoI restriction sites to construct expression vector pET-NM. c SDS-PAGE analysis of the recombinant protein NM expressed in E. coli BL21 (DE3) cells. Lane M for protein size markers (kDa), lanes U and I for total cellular proteins of E. coli before and after IPTG-induced protein expression, respectively, lanes S and P for the supernatants and pellets of the bacterial cell lysate after sonication, respectively. The theoretical molecular weight of NM is 38 kDa

Back to article page