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Fig. 5 | BMC Biotechnology

Fig. 5

From: Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin

Fig. 5

Cytotoxicity of recombinant immunotoxins towards CD64+ M1-like macrophages and CD64 HeLa cells. a PCR validation of CD64 expression. Lane 1, 1 kb plus DNA ladder; lane 2, CD64 expression in M1-like macrophage; lane 3, CD64 expression in HeLa cells; lane 4, GAPDH expression in M1-like macrophage; lane 5, GAPDH expression in HeLa cells. b, c, d Cytotoxicity of HALT-1 alone and recombinant immunotoxins was measured at increasing concentrations (0, 5, 10, 15, 20, 25 and 30 μg/mL) against CD64+ M1-like macrophages and CD64 HeLa cells. b HALT-1; c HALT-1-scFv; d scFv-HALT-1. Data are presented as mean ± standard deviations from triplicate experiments. See also Figure S3; Additional file 2

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