Fig. 1
From: Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin
12% SDS-PAGE image and binding assay of α-CD64 scFv. a Expression of recombinant α-CD64-scFv. Lane 1, 10–250 kDa protein ladder; lane 2, soluble fraction; lane 3, insoluble fraction. The expected band of 32 kDa was observed in the insoluble fraction. b α-CD64-scFv after refolding in a series of deceasing urea concentrations. Lane 1, protein ladder, lane 2, E. coli cell lysate with the induction of IPTG; lane 3, E. coli cell lysate without IPTG, and lane 4, refolded α-CD64 scFv visible as the band of 32 kDa. c ELISA assay of α-CD64 scFv binding against various concentrations of CD64 (0, 0.5, 1.0, 5.0 and 10 ng/mL). Maximum binding (0.2362) was achieved at 5 ng/mL of CD64. Each point is the mean generated from triplicate reactions. The controls (no scFv, no anti-CD64 Ab, and no anti-rabbit IgG-HRP) showed no absorbance signal. See also Figure S1; Additional file 2