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Table 5 glmM primers and probes sequences

From: An improved quantitative real-time polymerase chain reaction technology for Helicobacter pylori detection in stomach tissue and its application value in clinical precision testing

Category

Sequence (5′ → 3′)

Forward primer 1(F1)

GCTCTCACTTCCATAGGCTATAATG

Forward primer 2(F2)

GCTTTAACTTCCATAGGCTATAATGT

Reverse primer(R)

GCGCATGTCTTCGGTTAAAA

Probe 1(P1)

FAM-TAGGGCCTATGCCTACCCCTGCGA-HBQ1a

Probe 2 (P2)

FAM-TAGGGCCTATGCCCACCCCTGC-HBQ1

  1. aFAM is the fluorescence reporter, and HBQ1 is the fluorescence quencher