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Table 1 A comparison of the methanol-stable of EstGSu753 (this study) and Geobacillus stearothermophilus lipase T6/mutant at various methanol concentration

From: Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system

Items

t1/2 (min) (room temperature, 25 °C)

50% methanol

60% methanol

70% methanol

80% methanol

90% methanol

EstGSu753

33,300

14,400

11,640

3650

1980

lipase T6 [10]

347

173

4

T6: H86Y/A269T [10]

2429

1777

258