Skip to main content

Table 1 Efficiency of PDS mutagenesis with Km selection

From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Transformed organ

Strain

Vector

No. of regenerated plants

No. of tested plantsa

No. of mutants

Efficiencyb (%)

Tuber

gv2260

CP025

36

16

11

69

PROGED

24

8

7

88

LBA4404

CP025

56

21

18

86

PROGED

28

17

8

47

Leaf

gv2260

PROGED

24

14

9

64

LBA4404

16

9

7

78

  1. a Plants for testing the presence of mutation in the PDS gene were selected randomly
  2. b Efficiency (%) of PDS mutagenesis was calculated as number of mutants detected by the restriction enzyme site loss method in PCR amplification of genomic DNA after digestion with MlyI / number of tested plants × 100