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Fig. 2 | BMC Biotechnology

Fig. 2

From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Fig. 2

Detection of target mutations obtained with continuous Km selection in the PDS gene of potatoes by PCR assay using the primer pair PDS Fw and PDS R (Additional file 1: Table S1). a, 1–4, Detection of mutations in plants derived from tuber transformation with A. tumefiaciens LBA4404 (PROGED::gPDS); 5–9, detection of mutations in plants derived from tuber transformation with A. tumefiaciens gv2260 (PROGED::gPDS). Lane 9 shows the mutant PDS.5 m, the phenotype of which is shown in Fig. 3b. PCR was carried out with 36 cycles and fragments were separated on a 1% agarose gel. b, 1–5, Detection of mutations in plants derived from leaf transformation with A. tumefiaciens LBA4404 (PROGED::gPDS); 5–10, detection of mutations in plants derived from leaf transformation with A. tumefiaciens gv2260 (PROGED::gPDS). Lane 1 shows the mutant PDS.28 g, the phenotype of which is shown in Fig. 3c. PCR was carried out with 34 cycles and fragments were separated on a 1.2% agarose gel. M, DNA ladder; D, non-transformed ‘Désirée’; PDS, PCR fragments amplified from intact genomic DNA; MlyI-PDS, PCR fragments amplified from MlyI-digested genomic DNA

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