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Table 4 The cytokine levels of splenocytes extracted from mice after a lethal challenge with Legionella pneumophila

From: Recombinant PAL/PilE/FlaA DNA vaccine provides protective immunity against Legionella pneumophila in BALB/c mice

 

L.p-control

L.p-pcDNA3.1

L.p-PAL/PiLE/FLaA

TNF-α

12 h

28.464 ± 3.656

30.544 ± 3.160

66.426 ± 6.966***

24 h

63.061 ± 6.975

66.022 ± 7.707

142.812 ± 18.872***

48 h

74.966 ± 8.033

79.856 ± 8.523

155.792 ± 16.642***

72 h

71.350 ± 7.257

78.119 ± 8.014

176.366 ± 19.816***

IFNγ

12 h

17.882 ± 2.151

18.063 ± 1.958

168.855 ± 17.771***

24 h

42.829 ± 6.708

41.573 ± 6.185

259.489 ± 31.379***

48 h

27.588 ± 4.677

28.726 ± 5.612

361.732 ± 39.412***

72 h

32.878 ± 3.567

35.138 ± 4.788

442.975 ± 51.127***

IL-10

12 h

35.905 ± 5.921

38.285 ± 6.257

638.272 ± 72.853***

24 h

26.358 ± 2.762

25.189 ± 3.560

597.381 ± 70.460***

48 h

18.722 ± 2.776

18.359 ± 3.338

580.414 ± 71.515***

72 h

23.453 ± 4.065

22.517 ± 4.267

464.455 ± 47.797***

12 h

7.545 ± 0.848

7.866 ± 0.832

166.512 ± 19.016***

24 h

9.129 ± 1.099

9.616 ± 1.083

153.189 ± 16.667***

IL-4

48 h

16.399 ± 2.269

18.371 ± 2.041

180.543 ± 20.530***

72 h

19.566 ± 2.546

21.151 ± 3.770

182.311 ± 23.732***

12 h

32.346 ± 3.307

31.066 ± 3.123

187.781 ± 24.391***

24 h

25.581 ± 2.879

24.311 ± 2.506

263.204 ± 38.746***

IL-12

48 h

21.841 ± 2.484

22.015 ± 2.566

360.088 ± 60.515***

72 h

27.231 ± 3.618

27.220 ± 3.528

439.624 ± 50.110***

  1. ***, p < 0.001 versus the L.p-pcDNA3.1 group