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Fig. 4 | BMC Biotechnology

Fig. 4

From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Fig. 4

Phenotype of GD B. dothidea HTLW03 transformants. a Colony morphology of ΔBdo_05381–1, ΔBdo_05381–2, and ΔBdo_02540–1 in Petri plates containing PDA medium or PDA medium supplemented with 50 mg/L Congo red and 0.7 M NaCl. Accumulation of melanin in 6-day-old cultures is presented.b Comparison of the colony diameters of 60-h-old cultures on PDA medium. c Inhibitory effects of 50 mg/L Congo red and 0.7 M NaCl on the WT control and GD transformants. Bars represent the standard error of two independent experiments. The significance of the differences between the GD transformants (ΔBdo_05381–1, ΔBdo_05381–2, and ΔBdo_02540–1) and the WT control was analyzed based on an ANOVA and Duncan’s method. Different letters represent significant differences (P < 0.01)

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