Fig. 1From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothideaHomologous recombination strategy and primers used for verifying the Bdo_05381-disrupted transformants. Arrows indicate the positions of the primers used for constructing the plasmid and for verifying the transformants by PCR. The restriction enzyme digestion sites are marked. The hph gene controlled by the trpC promoter was used as the resistance marker. The 5′ and 3′ fragments flanking the target gene were linked to the sequences upstream and downstream of hph, respectively, to disrupt the target gene. A 500-bp hph gene probe was used for a Southern blot analysisBack to article page