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Fig. 7 | BMC Biotechnology

Fig. 7

From: Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies

Fig. 7

Characterization of polyclonal and monoclonal antibodies against rBacCPA250–363H6 by western blotting. The MAbs (section a-d) and PAbs (section e) generated were tested against culture supernatant of the C. perfringens CPA toxin producer. The name of the tested antibody is shown below the corresponding picture. The MW marker is shown on the left. Section a and b: Culture supernatant of C. pefringens strain C-5560/18 (lane 1), culture supernatant of C. septicum, used as a negative control (lane 2), and a mixture of full length PLC and rBacCPA250–363H6 (lane 3). Section c and d: Culture supernatant of C. perfringens strain C-5560/18 (lane 1), culture supernatant of C. septicum, used as a negative control (lane 2), full length PLC (lane 3), and rBacCPA250–363H6 (lane 4). Section e: Rabbit anti-rBacCPA250–363H6 PAbs and anti-PLC (Star Fish): mixture of rBacCPA250–363H6 and PLC (lane 1), different culture supernatants of C. perfringens CPA protein producers (lane 3–5), and culture supernatant of C. septicum, used as a negative control (lane 2). Section f: MAb 318F11B7 tested against recombinant and native CPA: mixture of rBacCPA250–363H6 and PLC (lane 1), different culture supernatants of C. perfringens CPA protein producers (lane 2–4), and culture supernatant of C. septicum, used as a negative control (lane 5)

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