Fig. 3
From: A self-aggregating peptide: implications for the development of thermostable vaccine candidates
The PH(1–110) GFP particles are purified and separated by size in a single centrifugation step. a Sucrose gradients (right) in which the separation of the particles is observed after centrifugation. With the SDS-PAGE (left) it can be seen that PH(1–110) GFP particles highly pure particles are recovered in each gradient. b Confocal microscopy shows the presence of PH(1–110) GFP particles of different sizes obtained in each sucrose gradient. c With the NTA equipment the sizes of the particles and the concentration of each particle were measured by 1 mL of solution. The peaks of the curves show the populations of particles. Dotted lines indicate the SD