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Table 2 Bacterial strains and plasmids used in this study

From: Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis

Strain or plasmidRelevant phenotypesReferences
Strains
E. coli Top 10 Invitrogen
L. lactis NZ9000MG1363 pepN::nisRK, host strain for all lactococcal plasmids[41]
L. lactis pNZintL. lactis harbouring pNZint plasmidThis study
Plasmid
pCRTM-Blunt II-TOPO®Zero Blunt® TOPO® PCR cloning KitInvitrogen
pUC192686 bps, AmpR, Cloning vectorNew England Biolabs Inc. (NEB)
pNZ80483348 bps, CmR, Nisin inducible expression vector[41]
pMG36e3611 bps, ErmR[42]
pNZSPKNucModified pNZ8048 carrying SPK1 signal peptide, nuc gene from S. aureusMicrobial Biotech Lab, UPM
pNZ170GusModified pNZ8008, PnisA was replaced with P170Microbial Biotech Lab, UPM
pNZintModified pNZ8048 carrying integrase gene of TP901–1This study
apS1Secretion integrative vector harboring attP of T901–1, P170, E. coli rep origin, USP45-LEISSTCDA, EmRThis study
apS2Secretion integrative vector harboring attP of T901–1, P170, E. coli rep origin, SPK1, EmRThis study
apS3Secretion integrative vector harboring attP of T901–1, PnisA, E. coli rep origin, USP45-LEISSTCDA, EmRThis study
apS4Secretion integrative vector harboring attP of T901–1, PnisA, E. coli rep origin, SPK1, EmRThis study
apSD1Surface display integrative vector harboring attP of T901–1, P170, E. coli rep origin, USP45-LEISSTCDA, PrtP anchor domain, EmRThis study
apSD2Surface display integrative vector harboring attP of T901–1, P170, E. coli rep origin, SPK1, PrtP344 anchor domain, EmRThis study
apSD3Surface display integrative vector harboring attP of T901–1, PnisA, E. coli rep origin, USP45-LEISSTCDA, PrtP344 anchor domain, EmRThis study
apSD4Surface display integrative vector harboring attP of T901–1, PnisA, E. coli rep origin, SPK1, PrtP344 anchor domain, EmRThis study
  1. a Plasmids had variations where nuclease reporters were cloned into the MCS and denoted with suufix-nuc in text