Table 2 Bacterial strains and plasmids used in this study

E. coli Top 10

Invitrogen

L. lactis NZ9000

MG1363 pepN::nisRK, host strain for all lactococcal plasmids

[41]

L. lactis pNZint

L. lactis harbouring pNZint plasmid

This study

pCRTM-Blunt II-TOPO®

Zero Blunt® TOPO® PCR cloning Kit

Invitrogen

pUC19

2686 bps, Amp^R, Cloning vector

New England Biolabs Inc. (NEB)

pNZ8048

3348 bps, Cm^R, Nisin inducible expression vector

[41]

pMG36e

3611 bps, Erm^R

[42]

pNZSPKNuc

Modified pNZ8048 carrying SPK1 signal peptide, nuc gene from S. aureus

Microbial Biotech Lab, UPM

pNZ170Gus

Modified pNZ8008, P_nisA was replaced with P₁₇₀

Microbial Biotech Lab, UPM

pNZint

Modified pNZ8048 carrying integrase gene of TP901–1

This study

^apS1

Secretion integrative vector harboring attP of T901–1, P₁₇₀, E. coli rep origin, USP45-LEISSTCDA, Em^R

This study

^apS2

Secretion integrative vector harboring attP of T901–1, P₁₇₀, E. coli rep origin, SPK1, Em^R

This study

^apS3

Secretion integrative vector harboring attP of T901–1, P_nisA, E. coli rep origin, USP45-LEISSTCDA, Em^R

This study

^apS4

Secretion integrative vector harboring attP of T901–1, P_nisA, E. coli rep origin, SPK1, Em^R

This study

^apSD1

Surface display integrative vector harboring attP of T901–1, P₁₇₀, E. coli rep origin, USP45-LEISSTCDA, PrtP anchor domain, Em^R

This study

^apSD2

Surface display integrative vector harboring attP of T901–1, P₁₇₀, E. coli rep origin, SPK1, PrtP₃₄₄ anchor domain, Em^R

This study

^apSD3

Surface display integrative vector harboring attP of T901–1, P_nisA, E. coli rep origin, USP45-LEISSTCDA, PrtP₃₄₄ anchor domain, Em^R

This study

^apSD4

Surface display integrative vector harboring attP of T901–1, P_nisA, E. coli rep origin, SPK1, PrtP₃₄₄ anchor domain, Em^R

This study