Fig. 2From: Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid productionVerification of mtpA gene disruption in the transformant a) Disruption of mtpA gene with bipartite substrates in A. carbonarius. b Southern blotting analysis. Lane 1 hydridization of the probe to the genomic DNA fragment (from the wild-type) digested by EcoRI and HindIII, Lane 2, DNA ladder, Lane 3, hydridization of the probe to the genomic DNA fragment (from mtpA-1 transformant) digested by EcoRI and HindIIIBack to article page