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Fig. 3 | BMC Biotechnology

Fig. 3

From: Use of the kojA promoter, involved in kojic acid biosynthesis, for polyketide production in Aspergillus oryzae: implications for long-term production

Fig. 3

Construction and confirmation of the ΔkojA_PkojA::wA and ΔkojA strains. (a) Change in the wA promoter region during construction of the ΔkojA_PkojA strain. (b) Construction of the ΔkojA_PkojA strain was confirmed by Southern hybridization. The site where the probe 1 hybridizes is shown. 1: ΔkojA, 2: ΔkojA_PkojA::wA. (c) Complementation of the niaD during construction of the ΔkojA strain. (d) Construction of the ΔkojA strain was confirmed by Southern hybridization. The site where the probe 2 hybridizes is shown. 3: ΔkojA_niaD-, 4: ΔkojA

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