Table 1 Agreement analysis between direct PCR sequencing and stuntmer PCR
Exon 21 L858R | Traditional PCR | Total | ||
Positive | Not found | |||
FFPE | ||||
 Stuntmer PCR | ||||
  Positive | 291 | 56 | 347 | |
  Not found | 0 | 935 | 935 | |
 Total | 291 | 991 | 1282 | |
Pleural effusion | ||||
 Stuntmer PCR | ||||
  Positive | 59 | 33 | 92 | |
  Not found | 0 | 226 | 226 | |
 Total | 59 | 259 | 318 | |
Total | 350 | 1250 | 1600 | |
Positive percent agreement (95% CI) | 100% (98.9, 100%) | |||
Negative percent agreement (95% CI) | 92.9% (91.3, 94.2%) | |||
Overall percent agreement (95% CI) | 94.4% (93.2, 95.5%) | |||
Exon 19 deletion | Traditional PCR | Total | ||
Deletion | Insertion | Not found | ||
FFPE | ||||
 Stuntmer PCR | ||||
  Deletion | 272 | 0 | 140 | 412 |
  Not found | 0 | 0 | 870 | 870 |
 Total | 272 | 0 | 1010 | 1282 |
Pleural effusion | ||||
 Stuntmer PCR | ||||
  Deletion | 56 | 0 | 55 | 111 |
  Insertion | 0 | 1 | 0 | 1 |
  Not found | 0 | 0 | 206 | 206 |
 Total | 56 | 1 | 261 | 318 |
Total | 328 | 1 | 1271 | 1600 |
Positive percent agreement (95% CI) | 100% (98.8, 100%) | |||
Negative percent agreement (95% CI) | 84.7% (82.6, 86.5%) | |||
Overall percent agreement (95% CI) | 87.8% (86.1, 89.3%) | |||
Exon 20 T790 M | Traditional PCR | Total | ||
Positive | Not found | |||
FFPE | ||||
 Stuntmer PCR | ||||
  Positive | 16 | 32 | 48 | |
  Not found | 0 | 1234 | 1234 | |
 Total | 16 | 1266 | 1282 | |
Pleural effusion | ||||
 Stuntmer PCR | ||||
  Positive | 2 | 8 | 10 | |
  Not found | 0 | 308 | 308 | |
 Total | 2 | 316 | 318 | |
Total | 18 | 1582 | 1600 | |
Positive percent agreement (95% CI) | 100% (82.4, 100%) | |||
Negative percent agreement (95% CI) | 97.5% (96.6, 98.1%) | |||
Overall percent agreement (95% CI) | 97.5% (96.6, 98.2%) | |||