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Fig. 3 | BMC Biotechnology

Fig. 3

From: A new primer construction technique that effectively increases amplification of rare mutant templates in samples

Fig. 3

Different types of mutations in the same region can be amplified by the same stuntmer. The EGFR S768 stuntmer was able to selectively amplify the point mutation (a) and the insertion mutation (b) in EGFR exon 20. The stuntmer designed to detect deletions in exon 19 was able to detect multiple types of deletion mutations (c) as well as an insertion mutation (d). In all cases, the sequencing results indicated that the number of mutant templates exceeded the number of wild-type templates in stuntmer PCRs

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BMC Biotechnology

ISSN: 1472-6750

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