Fig. 2From: A new primer construction technique that effectively increases amplification of rare mutant templates in samplesMutant sequence enrichment using stuntmer PCR. The abilities of stuntmers to detect four different mutation scenarios (single nucleotide mutation, two different single nucleotide mutations, and a deletion mutation) were tested under three different sample conditions (only wild-type, 90:10 ratio of wild-type:mutant, and 99:1 ratio of wild-type:mutant). In all cases, the stuntmers were able to detect the mutations even when the mutant templates were present in only 1% of the tested samples.The wild-type template was completely inhibited in the T790M point mutation (a) and exon 19 deletion mutation (b). The arrow indicates where the deletion mutation occurredBack to article page