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Fig. 2 | BMC Biotechnology

Fig. 2

From: A new primer construction technique that effectively increases amplification of rare mutant templates in samples

Fig. 2

Mutant sequence enrichment using stuntmer PCR. The abilities of stuntmers to detect four different mutation scenarios (single nucleotide mutation, two different single nucleotide mutations, and a deletion mutation) were tested under three different sample conditions (only wild-type, 90:10 ratio of wild-type:mutant, and 99:1 ratio of wild-type:mutant). In all cases, the stuntmers were able to detect the mutations even when the mutant templates were present in only 1% of the tested samples.The wild-type template was completely inhibited in the T790M point mutation (a) and exon 19 deletion mutation (b). The arrow indicates where the deletion mutation occurred

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