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Table 2 List of primers used for the construction of tethered eCG mutants

From: Characterization of tethered equine chorionic gonadotropin and its deglycosylated mutants by ovulation stimulation in mice

 

Primer name

Location

Primer Sequence

1

eCGβ/α β-subnuit

-

5′- TGAATTCACCATGGAGACGGTCCAG -3′

Eco R I site

2

myc eCGβ/α reverse

-

5′- TCCATCAGGAAAAGAAGTCTTTATTGG -3′

3

myc eCGβ/α forward

-

5′- ATAAAGACTTCTTTTCCTTGATGGAGAG -3′

4

eCGβ/αΔ56 reverse

α 56

5′- TGAGGTGATCTGCTTTGGGACCAACAT -3′

5

eCGβ/αΔ56 forward

α 56

5′- ATGTTGGTCCCAAAGCAGATCACCTCA -3′

6

eCGβ/αΔ82 reverse

α 82

5′- GGCCTCCTTCTCAGCGGCCAGAGTGGCGTTGAT -3′

7

eCGβ/αΔ82 forward

α 82

5′- ATCAACGCCACTCTGGCCGCTGAGAAGGAGGCC -3′

8

eCGβΔ13/α reverse

β 13

5′- AGCAGCCAGAGTGGCGTTGATGGGCCGGCACAG -3′

9

eCGβΔ13/α forward

β 13

5′- CTGTGCCGGCCCATCAACGCCACTCTGGCTGCT -3′

10

eCGβ-D/α reverse

β : 121-149

5′- TCCATCAGGAAAGGCCTGGGGGGCACAGGC -3′

11

eCGβ-D/α forward

β : 121-149

5´- GCCCCCCAGGCCTTTCCTCATGGAGAGTTT -3´

12

eCGβ/α α-subunit

-

5′- CCGTCGACTTTAAATCTTGTGGTGATAGCA -3′

Sal I site