Fig. 2From: Characterization of tethered equine chorionic gonadotropin and its deglycosylated mutants by ovulation stimulation in miceQuantitative analysis of rec-eCG mutants and myc-tagged rec-eCG mutants by ELISA after transient transfection into CHO-K1 cells. a Quantities of dimeric rec-eCG mutants were measured by an ELISA. b Tethered rec-eCG mutants were detected. c Quantities of myc-tagged rec-eCG mutants were analyzed by an ELISA. The myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) was added between the first and second amino acid residue of the β-subunit of the mature eCG protein. Values with different superscripts are significantly different (p < 0.05)Back to article page