Fig. 6From: Lack of specificity associated with using molecular beacons in loop mediated amplification assaysFluorescent signal from MB-LAMP with looped or linear DNA.35Sp oligonucleotide designed for ‘dumbbell’ looped structure undiluted (1A) and ten-fold dilution (1B) in blue, NTC in orange. Fluorescence from LBP molecular beacon in real-time at 60 degrees C for 5 min and melt curve analysis. Average fluorescence and melt temperature derived from replicates. 35Sp oligonucleotide of the same length without loops undiluted (2A) and ten-fold diluted (2B) in blue with NTC in orange. Fluorescence acquisition from LBP molecular beacon at 60 degrees C for 5 min and melt curve analysisBack to article page