Fig. 2From: Lack of specificity associated with using molecular beacons in loop mediated amplification assaysLAMP amplification with 35Sp LoopF molecular beacon. LAMP primers designed to amplify part of the 35S promoter sequence detected by fluorescence from a LAMP molecular beacon targeting the LoopF sequence. 1A shows the fluorescence for dilutions of the 35Sp LAMP MB; 125 nanomolar dark blue, 12.5 nanomolar light blue, 1.25 nanomolar light green, 125 picomolar pink and NTC in orange. 2A denotes the fluorescent output against cycles of 1 min at 63 degrees C for 35Sp LAMP amplification excluding the LoopF oligonucleotide (positive samples with template in blue and NTC in orange). 3A charts the fluorescent output against cycles of 1 min at 63 degrees C for 35Sp LAMP amplification with the inclusion of the LoopF oligonucleotide. LAMP molecular beacon used at a concentration of 0.32 micromolar for these two assays. Melt curve analysis shown in 1B to 3B with peaks invertedBack to article page