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Table 3 Primer sequences for LASSO assembly and capture

From: Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

Primer

Sequence (5′-3′)

BlaF

GAGTATTACCGCGGCGAATTC

RFPR200EcoRI

AAGCTGGAATTCGCTTCCGTACTGGAACTGAGGGC

SapINew

GGTTCCTGGCTCTTCGATC

TiolNew

A*T*C*GCCGCAAGAAGTGTU

PCR1kbCaptF400

GTGAAACTCAGAGGAACCAACTTCC

ICeul200CaptF

CTCCCCTTCTCCTGGGATATTCTG

M13_137_R(Tiol)

TTCAAAGCGAACCAGACCGG

M13_137_F(SapINew)

/5Phos/GCAATCCGCTTTGCTTCTGAC

  1. “*” symbol denotes phosphorothioate bonds while /5Phos/ indicates a phosphorylated 5′ end