Fig. 5
From: Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
Possible ligation events within probe assembly. Both Probe A and Probe B are formed after the Fusion PCR step prior to restriction enzyme digest and self-circularization. Examples of intermolecular ligation errors formed during self-circularization step (ligation reaction) of the LASSO probe assembly. Resultant discordant probes from intermolecular ligations shown here have mismatched arms from separate probe sequences