Fig. 4
From: Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
Single-Target LASSO capture gel resolvability. a Single target captures resolved on agarose (left) and polyacrylamide gels (right). Both gels are scaled to PAGE DNA ladder, with the major band slightly underneath the 300 bp ladder band. For control lanes, GDNA only control only contains M13 genomic DNA while LASSO only controls only contain M13_137 probes. Annealing temperatures for captures are denoted by “Ta”, while gDNA and LASSO probe only controls were done at 60 °C b Pixel density plots of single target LASSO captures on agarose and PAGE were obtained by ImageJ plot lane functions. In both plots, LASSO captures (60 °C Ta) are overlaid with a LASSO probe only control to compare non-specific capture with baseline pixel density