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Table 3 PCR primers used in the present study

From: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Primer name

Primer sequence (5′ → 3′)

P1

ACACTCGAGaaaagaGACTCCGACGACAGGGTCAC

P2

TATGCGGCCGCTCAATGGTGATGGTGATGATGCGGCCAGCCCTGC

P3

ACACTCGAGaaaagaGACAAT GGC GCG GGG GAAG

P4

TATGCGGCCGCTCAATGGTGATGGTGATGATGGGGAGCCCGGAACG

P5

TATGCGGCCGCCAGCTTTCTAGAACAAAAACTCATCTC

P6

CTTAAATATTAGGAAAAACGGTAACCTTATCTCACTTAATCTTCTGTACTCTG

P7

CAGAGTACAGAAGATTAAGAGAGATAGTTAGGTTACCGTTTTTCCTAATATTTAAG

P8

CGCGGATCCCTTCCACCAACAGTCAACCACCAGTC

P9

GGTATTAACGGTTTCGGACGTATTG

P10

GATGTTGACAGGGTCTCTCTCTTGG

P11

TGAAGAAAGAATTGGCTAACGG

P12

AGCTGGTCTGAAAGCATCTGG

  1. Remarks: The underlined sites are those for the digestion of restriction enzymes XhoI. The wavy line sites are those for the digestion of restriction enzymes NotI. The double corrugated underlined sites are the ones for the digestion of restriction enzymes BamHI. 6xHis-tag label sequence is indicated by the dotted line. The Kex2-endopeptidase recognition site is marked with lowercase letters. The bold underlining represents the sequence of overlapping segments of the AOX1 terminator-rDNA fusion gene
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BMC Biotechnology

ISSN: 1472-6750

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