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Table 1 Predicted solubilities of recombinant human erythropoietin. Solubility profile was defined as described in Chan et al. [29]. Positive patch sizes are divided by that best separating soluble and insoluble datasets [33], above 1.0 implies predicted insolubility

From: Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation

Protein

Pos patch ratio to threshold

Prediction

rHuEPO wild-type

1.49

Insoluble

rHuEPO F48D

0.75

Soluble

rHuEPO R150D

0.61

Soluble

rHuEPO F48D/R150D

0.47

Soluble

rHuEPO E13K

2.47

Insoluble