Fig. 2

Western blot of rHuEPO expression and solubility. (a-b) Equal volumes of total protein and soluble fraction were probed with Mouse anti-polyHis antibody and imaged with the Odyssey Imaging System for BL21 (DE3) pLysS (a) and SHuffle (b) strains. (c) Experimental solubility was determined by the distribution of rHuEPO between soluble and inclusion body fraction in E. coli. (d) Relative total rHuEPO production (arbitrary units). Error bars represent the + SEM for measurements in triplicate; statistical significance was calculated using a two-sided unpaired t-test (*P < 0.05, ** P < 0.01). BL21 (DE3) pLysS (■); SHuffle (□)