Table 2 Analysis of targeted gene mutations induced by CRISPR-Cas9 cassettes in transgenic soybean lines. Transgenic plants containing confirmed FAD2–2 mutagenesis were subjected to look for off target editing
SgRNA | No. of putative off target sites | No. of loci of putative off target sites | No. of examined loci | No. of plants examined | No. of off target mutation |
|---|---|---|---|---|---|
FAD2–2-sgRNA2 | 20 | 19 | 12 | 8 | 0 |