Fig. 4

Detection of targeted mutation in transgenic soybean plants using CRISPR-Cas9 system. a PCR Detection of Cas9 gene in different soybean transgenic plants. We used plasmid DNA (P) as positive control and wild type DNA as negative (WT). The Series of 1 to 8 express different soybean transgenic plants. b The PCR product analysis of target gene (FAD2–2) of the independent transgenic plants, which is successfully cloned and subjected to sequence analysis for mutation detection. Sequences analysis revealed efficient targeted genome editing using CRISPR-Cas9 system. Red letters in the sequence indicate mutation where, S: indicate substitution, +: represent addition or insertion while/−: indicate deletion. The letters on right side show the number of transgenic plants where the mutations occurred in transgenic lines with the same type. . c Sequencing chromatograms of soybean transgenic plant. The blue color alphabets represent nucleotide substitution