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Fig. 2 | BMC Biotechnology

Fig. 2

From: Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis

Fig. 2

Influence of rising nisin levels and temperature 30 °C (panel a), growing nisin levels and temperature 20 °C (panel b), and induction at OD600 (panel c) at various time points on the expression of rE7 proteins in L. lactis containing pNZ8123-HPV16-optiE7 (Left) and pNZ8123-HPV16-E7 (right). Panel a and b: Induction time with 1 ng/mL (diamond), 5 ng/mL (square), 10 ng/mL (triangles), 15 ng/mL (multiple), and 20 ng/mL (star) nisin. Panel c (at 20 °C): induction at OD600 = 0.5 (diamond), OD600 = 0.6 (square), OD600 = 0.7 (triangle), OD600 = 0.8 (multiple)

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